Expression of a cold-adapted fish trypsin in
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چکیده
منابع مشابه
A novel cold-adapted imidase from fish Oreochromis niloticus that catalyzes hydrolysis of maleimide.
In this paper we report the first comparative study of cold-adapted imidase (EC 3.5.2.2) from the fish (Oreochromis niloticus) liver and its thermophilic counterparts taken from pig liver and Escherichia coli (overexpressed recombinant hydantoinase from Agrobacterium radiobacter NRRL B1). Approximately 6000-fold purification and a 40% yield of fish imidase activity were obtained through ammoniu...
متن کاملCold-adapted Antarctic fish: the discovery of neuroglobin in the dominant suborder Notothenioidei.
Novel globins, such as neuroglobin (Ngb) and cytoglobin, have recently been discovered in many vertebrates. Ngb is mainly expressed in neurons and plays a neuroprotective role during hypoxic stress. Neuronal hypoxia and cerebral ischemia induce Ngb expression; knocking down Ngb expression increases hypoxic neuronal injury in vitro and ischemic cerebral injury in vivo. Although Ngb was originall...
متن کاملDevelopment and Sequence Analysis of a Cold-Adapted Strain of Influenza A/New Caledonia/20/1999(H1N1) Virus
Background and Aims: Vaccination is the most effective method to prevent influenza infection. Among the available vaccines, cold-adapted live-virus vaccines are suitable approach that have been produced and evaluated for recent years in few countries. The goal of this project was to derivate a cold adapted variant of the influenza A/New Caledonia/20/1999(H1N1). Materials and Methods: Influenza ...
متن کاملBehavioral buffering of global warming in a cold‐adapted lizard
Alpine lizards living in restricted areas might be particularly sensitive to climate change. We studied thermal biology of Iberolacerta cyreni in high mountains of central Spain. Our results suggest that I. cyreni is a cold-adapted thermal specialist and an effective thermoregulator. Among ectotherms, thermal specialists are more threatened by global warming than generalists. Alpine lizards hav...
متن کاملCold Adaptation, Ca2+ Dependency and Autolytic Stability Are Related Features in a Highly Active Cold-Adapted Trypsin Resistant to Autoproteolysis Engineered for Biotechnological Applications
Pig trypsin is routinely used as a biotechnological tool, due to its high specificity and ability to be stored as an inactive stable zymogen. However, it is not an optimum enzyme for conditions found in wound debriding for medical uses and trypsinization processes for protein analysis and animal cell culturing, where low Ca(2+) dependency, high activity in mild conditions and easy inactivation ...
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2005
ISSN: 1567-1356
DOI: 10.1016/j.femsyr.2005.02.007